Discover how PIPS revolutionizes root canal disinfection by significantly reducing E. faecalis, even at varying NaOCl concentrations and laser settings.
Authors: Golob BS, Olivi G, Vrabec M, El Feghali R, Parker S, Benedicenti S
Journal: Journal of Endodontics
Objective
The primary objective of this study was to evaluate the effectiveness of photon-induced photoacoustic streaming (PIPS) using an Er:YAG laser in reducing Enterococcus faecalis within root canals. The study examined various laser settings and sodium hypochlorite (NaOCl) concentrations to determine the optimal parameters for maximum bacterial reduction.
By exploring different configurations, the research aimed to establish whether PIPS could enhance the antibacterial efficacy of NaOCl and improve overall root canal disinfection.
Materials and Methods
The study involved the use of extracted single-rooted human teeth which were sterilized and subsequently inoculated with Enterococcus faecalis. These samples were incubated to allow for biofilm formation, mimicking real-world conditions within root canals.
Teeth were randomly assigned to groups based on varying PIPS laser parameters and NaOCl concentrations. PIPS was performed using a subablative Er:YAG laser tip, which generated rapid photoacoustic shockwaves to activate the irrigant throughout the canal system. The bacterial reduction was measured by culturing samples before and after the irrigation process.
Key Results
- PIPS significantly reduced E. faecalis counts compared to conventional irrigation.
- The combination of PIPS with NaOCl achieved the highest level of bacterial inhibition, with some studies reporting up to 100% inhibition under optimal conditions.
- PIPS was effective even at lower NaOCl concentrations and in minimally prepared canals, indicating efficient irrigant distribution and biofilm disruption.
Conclusion
Laser-activated irrigation using 5% NaOCl and a modified PIPS protocol resulted in effective eradication of the bacterial biofilm and removal of the smear layer.
Overall, PIPS represents a promising advancement in root canal therapy, offering enhanced disinfection capabilities, even in difficult-to-reach areas of the canal system. Its ability to perform effectively at lower NaOCl concentrations also underscores its potential for safer and more efficient endodontic treatments.
ACCESS THE FULL ARTICLE HERE
